Ch 4 DNA Websites
DNA
Replication demo
http://207.207.4.198/pub/flash/24/menu.swf
http://www.ncc.gmu.edu/dna/repanim.htm
http://medlab.lzu.edu.cn/image/flash/4.swf
http://library.thinkquest.org/27819/cgi-bin/quiz.cgi?quiz=6_5
http://www.funtrivia.com/playquiz/quiz18993515bfd10.html
Transcription
http://www.ncc.gmu.edu/dna/mRNAanim.htm
http://www.stolaf.edu/people/giannini/flashanimat/molgenetics/transcription.swf
http://library.thinkquest.org/27819/cgi-bin/quiz.cgi?quiz=6_6
http://student.ccbcmd.edu/~gkaiser/biotutorials/protsyn/tsc_quiz.html
Transcription and translation
http://learn.genetics.utah.edu/units/basics/transcribe/
http://library.thinkquest.org/27819/cgi-bin/quiz.cgi?quiz=6_8
http://www.cst.cmich.edu/users/Benja1dw/BIO101/tools/quiz/dnarna.htm
http://www.johnkyrk.com/DNAtranslation.html
http://www.stolaf.edu/people/giannini/flashanimat/molgenetics/translation.swf
http://207.207.4.198/pub/flash/26/transmenu_s.swf
http://www.ncc.gmu.edu/dna/ANIMPROT.htm
Karyotyping
http://bluehawk.monmouth.edu/~bio/karyotypes.htm
Use the following website to look at the pictures in the next section until I figure out how to upload them.
http://www.abbysenior.com/biology/dna_protein_synthesis.htm
Notes and Quiz
DNA
1. The Chemical makeup of DNA and RNA -
a. DNA – is made up of many DNA nucleotides (nucleotides are the building blocks of nucleic acids). Each DNA nucleotide is composed of deoxyribose (a pentose, or five carbon sugar), a phosphate molecule and one of four nitrogenous bases: Adenine, Thymine, Cytosine and Guanine. (A, T, C, G). The picture below shows the double stranded DNA structure and complementary bases of the DNA bases.
b. RNA – is made up of many RNA nucleotides (nucleotides are the building blocks of Nucleic acids). Each RNA nucleotide is composed of ribose (a pentose, or five carbon sugar), a phosphate molecule and one of four nitrogenous bases Adenine, Uracil, Cytosine and Guanine. (A, U, C, G).
c DNA and RNA Nucleotide Diagrams
The diagrams below show different diagrams of nucleotides, both RNA and DNA. Be able to recognize the sugar, the five sided figure in the first two diagrams and the five sided figure labeled Y in the third diagram, the phosphate (the circle with the P or Z inside) and the base (the six sided figure with the N in its structure, labeled W or X in the third diagram) The last diagram shows 4 nucleotides, showing bases, sugars and phosphates
2. DNA Replication - DNA is copied exactly to produce DNA. The diagram below shows the process
Steps in DNA Replication
a. Unzipping - the molecule of DNA is unzipped. The hydrogen bonds between the complementary base pairs are broken. Helicase is the enzyme which cause the DNA molecule to unzip. The DNA bases are exposed so complementary base pairing can occur. See the diagram of unzipping below.
b. Complementary base pairing - The nucleotide bases that make up the 2 strands of the DNA molecule are exposed and nucleotides located in the nucleus move into place by complementary base pairing. eg. Adenine is complementary to Thymine and vice versa, so A only bonds with T and T only bonds with A. Cytosine is complementary to Guanine and vice versa so C only bonds with G and G only bonds with C. The pairs of complementary nucleotides form the cross bridges of the DNA molecule (W and X in the diagram below). (the ladder steps)
In Transcription it is the same process only RNA is produced. (Uracil instead of Thymine).
See the diagrams of complementary base pairing below.
c. Joining of adjacent nucleotides - Using the diagram above, the vertical part (up and down) of the DNA molecule is formed when the sugar part (labelled Y) of one nucleotide bonds with the phosphate part (labelled Z) of an adjacent nucleotide. These bonds produce the sides of the ladder. The joining of the sugar/phosphate parts of adjacent nucleotide molecules causes the length of the new DNA molecule to increase. Two complete molecules of DNA are formed, identical to the original one. The diagram below shows how the sugar and the phosphate join.
3. Protein Synthesis – transcription then translation
A) Transcription - is the copying of the DNA molecule by mRNA
The 3 steps in Transcription: unzipping, Complementary base pairing and Joining of adjacent nucleotides
i. Unzipping
-A segment of DNA unzips. An enzyme (helicase) breaks the hydrogen bonds between the nitrogenous bases.
ii. Complementary Base Pairing
-RNA nucleotides bind with DNA nucleotides by complementary base pairing on one of the two DNA strands that are exposed after unzipping.
iii. Joining of Adjacent Nucleotides
An enzyme catalyzes the reaction that joins the sugar/phosphate molecules of adjacent nucleotides together.
-The newly produced mRNA strand passes from the nucleus into the cytoplasm to join with a ribosme.
B) Translation - mRNA codons are read, amino acids are brought to the mRNA strand and polypeptides (proteins) are produced at the ribosomes. Translation ends with the production of a protein.
The three specific parts of translation are:
i. Initiation - the ribosomal RNA (ribosome) and the mRNA strand become attached.
ii. Elongation - the polypeptide lengthens one amino acid at a time
-the tRNA anti-codons (3 letter code of the tRNA) bond to the mRNA codons (3 letter code of the mRNA) by complementary base pairing at the ribosome. The codons determine the type of amino acid brought to the polypeptide chain.
-The ribosome is large enough to accomodate two tRNA molecules at a time, one incoming and one outgoing The amino acid on the incoming tRNA forms a peptide bond with the amino acid on the outgoing tRNA.
-The ribosome then moves laterally, allowing the outgoing tRNA to leave and making space for a new incoming tRNA.
iii. Termination - occurs at the stop codon on the mRNA. The completed polypeptide dissociates from the ribosome
-Translation is shown in the diagram below. Be able to label the ribosomes (they look like snowmen, the mRNA strand (labeled X) and the polypeptide strand.
4. Determine the amino acids coded for by a strand of DNA
1. Determining codons from a strand of DNA
Consider the following strand of DNA read from left to right:
T T A T G C T C C T A A
When complementary base pairing occurs the following strand of mRNA is obtained.
A A U A C G A G G A U U
The codons are read three letters at a time from the transcribed mRNA. The codons are:
A A U A C G A G G A U U
2. Determining amino acids from mRNA codons
Find the codons in the table of mRNA codons below and the amino acids they code for.
AAU -Asparagine ACG - Threonine AGG - Arginine AUU -isoleucine
5 steps in protein synthesis
-DNA serves as a code for proteins
-Transcription occurs. One strand of DNA serves as a template (blueprint) for the formation of mRNA.
-after transcription, the newly formed mRNA goes into the cytoplasm and attaches to a ribosomes.
-tRNA which carry specific amino acids, have anticodons which bind complementarily with the mRNA codons
-The ribosome moves along the mRNA strand reading each codon. The tRNA anticodon bonds to it complementary codon. The amino acid carried by the tRNA is bonded to the polypeptide. The ribosome moves down the mRNA strand one codon at a time releasing a tRNA as it does and making room for another tRNA carrying an amino acid to join the mRNA.
a. DNA - carries the blueprint for the order of amino acids to be produced during protein synthesis.
b. Messenger RNA (mRNA) - copies the DNA molecule in the nucleus during transcription and goes into the cytoplasm and attaches itself to a ribosome. One 3 letter unit in an mRNA chain is called a codon.
c. Transfer RNA (tRNA) - drops off amino acids it is coded to carry at the ribsome. The 3 letter code is complementary to mRNA and this 3 letter code is called the anti-codon
d. Ribosome (rRNA) - this is where protein synthesis occurs. (Where the protein is assembled)
Recombinant DNA- contains DNA from 2 different sources. Organisms that have had a foreign gene inserted into them are called transgenic organisms
Use of Recombinant DNA research
a. in Bacteria - used to produce large amounts of protein, eg insulin, human growth hormone, and hepatitis vaccine.
b. in plants - genes transferred to cotton, corn, and potato strains have made these plants resistant to pests, because these cells produce an insect toxin.
-Plants have also been engineered to produce, hormones, clotting factors and antibodies
c. in animals. - genepharming using transgenic organisms to produce pharmaceuticals eg genes that code for therapeutic and diagnostic proteins are incorporated in and animal's DNA and appear in milk. There are plans to produce drugs for the treatment of cystic fibrosis, cancer, blood diseases, and other disorders. Antithroujmbi III is used for preventing blood clots in surgery is being produced by a herd of goats
DNA QUIZ QUESTIONS
1. What is the general name for chemicals like DNA and RNA
2. What general shape does the DNA molecule have?
3. List 4 differences between RNA and DNA
4. Describe the function of the mRNA in Protein Synthesis
5. What is the definition of a codon
6. What is the definition of an anti-codon
7. How many amino acids will result from the following strand of DNA?
A C G C C C A A A T A C
8. Name the two stages that make up protein synthesis
9. Where in the cell does replication take place?
10. Describe the function of the tRNA in Protein Synthesis?
11. Describe briefly the events that occur during transcription?
12. Name 2 common environmental mutagens
13. Describe briefly the events that occur during translation?
14. What is the definition of a) translation b) transcription
16. Define complementary base pairing and give an example?
17. Describe the function of the ribosome during protein synthesis
18. From a given strand of DNA. Show the results of Transcription or Replication
19. Make a drawing of DNA or RNA nucleotide and label the parts
20. Give 2 examples of a) purines b) pyrimidines
21. Name the 4 bases that make up DNA or RNA molecules
22. Describe how an environmental mutagen could cause a mutation
23. Describe briefly the events that occur during replication?
24. List 3 functions of DNA
25. Describe the function of the DNA in Protein Synthesis
26. What is the definition of a chromosomal mutation
27. What is the definition of a gene mutation
28. If the Nucleic acids are like ladders: What chemicals form the backbone of DNA or RNA molecules?
29. If the Nucleic acids are like ladders: What chemicals form the rungs of DNA or RNA molecule?
30. Briefly describe what occurs during Protein synthesis
31. What is recombinanant DNA. Give two uses of recombinant DNA
DNA SUBJECTIVE QUESTIONS
1. Describe 2 differences between the following pairs of terms
a. codon and anti codon b. replication and transcription c. RNA and DNA
d. transcription and translation e. chromosomal and gene mutation f. mRNA and tRNA
g. purine and pyrimidine h. complementary base pairing and joining of adjacent nucleotides
2. Describe complementary base pairing. Show an example.
3. Explain the roles/function of the following during Protein Synthesis:
a. DNA in the nucleus b. Transfer RNA (tRNA) c. Messenger RNA (mRNA) d. Ribosome
4. Name 2 environmental mutagens and describe how they could cause a mutation in an organism
5. Explain the process of DNA replication
6. Make a drawing of an RNA or DNA nucleotide and label the parts
7. Compare DNA and RNA with respect to the following things:
a. shape b. chemical makeup c. function d. abundance in the cell
8. Explain the process of translation
9. Explain the process of transcription
10. Describe in sequence, the process of protein synthesis. Include in your answer the names of the various steps, the organelles involved, and the names of the major molecules.
11. Describe how radiation or another such substance could cause mutations to occur.
12. From a given strand of DNA be able to determine the sequence of mRNA codons, and the amino acid strand produced.
Hot sheet questions
1. Describe the structure of DNA and RNA
2. Describe the steps in replication and transcription
3. Describe the function of the following during protein synthesis:
a. DNA b. mRNA c. tRNA d. rRNA e. ribosomes
4. Explain the process of Transcription
5. Briefly explain the process of translation
6. Describe the differences between RNA and DNA
7. Outline the process of protein synthesis
Replication demo
http://207.207.4.198/pub/flash/24/menu.swf
http://www.ncc.gmu.edu/dna/repanim.htm
http://medlab.lzu.edu.cn/image/flash/4.swf
http://library.thinkquest.org/27819/cgi-bin/quiz.cgi?quiz=6_5
http://www.funtrivia.com/playquiz/quiz18993515bfd10.html
Transcription
http://www.ncc.gmu.edu/dna/mRNAanim.htm
http://www.stolaf.edu/people/giannini/flashanimat/molgenetics/transcription.swf
http://library.thinkquest.org/27819/cgi-bin/quiz.cgi?quiz=6_6
http://student.ccbcmd.edu/~gkaiser/biotutorials/protsyn/tsc_quiz.html
Transcription and translation
http://learn.genetics.utah.edu/units/basics/transcribe/
http://library.thinkquest.org/27819/cgi-bin/quiz.cgi?quiz=6_8
http://www.cst.cmich.edu/users/Benja1dw/BIO101/tools/quiz/dnarna.htm
http://www.johnkyrk.com/DNAtranslation.html
http://www.stolaf.edu/people/giannini/flashanimat/molgenetics/translation.swf
http://207.207.4.198/pub/flash/26/transmenu_s.swf
http://www.ncc.gmu.edu/dna/ANIMPROT.htm
Karyotyping
http://bluehawk.monmouth.edu/~bio/karyotypes.htm
Use the following website to look at the pictures in the next section until I figure out how to upload them.
http://www.abbysenior.com/biology/dna_protein_synthesis.htm
Notes and Quiz
DNA
1. The Chemical makeup of DNA and RNA -
a. DNA – is made up of many DNA nucleotides (nucleotides are the building blocks of nucleic acids). Each DNA nucleotide is composed of deoxyribose (a pentose, or five carbon sugar), a phosphate molecule and one of four nitrogenous bases: Adenine, Thymine, Cytosine and Guanine. (A, T, C, G). The picture below shows the double stranded DNA structure and complementary bases of the DNA bases.
b. RNA – is made up of many RNA nucleotides (nucleotides are the building blocks of Nucleic acids). Each RNA nucleotide is composed of ribose (a pentose, or five carbon sugar), a phosphate molecule and one of four nitrogenous bases Adenine, Uracil, Cytosine and Guanine. (A, U, C, G).
c DNA and RNA Nucleotide Diagrams
The diagrams below show different diagrams of nucleotides, both RNA and DNA. Be able to recognize the sugar, the five sided figure in the first two diagrams and the five sided figure labeled Y in the third diagram, the phosphate (the circle with the P or Z inside) and the base (the six sided figure with the N in its structure, labeled W or X in the third diagram) The last diagram shows 4 nucleotides, showing bases, sugars and phosphates
2. DNA Replication - DNA is copied exactly to produce DNA. The diagram below shows the process
Steps in DNA Replication
a. Unzipping - the molecule of DNA is unzipped. The hydrogen bonds between the complementary base pairs are broken. Helicase is the enzyme which cause the DNA molecule to unzip. The DNA bases are exposed so complementary base pairing can occur. See the diagram of unzipping below.
b. Complementary base pairing - The nucleotide bases that make up the 2 strands of the DNA molecule are exposed and nucleotides located in the nucleus move into place by complementary base pairing. eg. Adenine is complementary to Thymine and vice versa, so A only bonds with T and T only bonds with A. Cytosine is complementary to Guanine and vice versa so C only bonds with G and G only bonds with C. The pairs of complementary nucleotides form the cross bridges of the DNA molecule (W and X in the diagram below). (the ladder steps)
In Transcription it is the same process only RNA is produced. (Uracil instead of Thymine).
See the diagrams of complementary base pairing below.
c. Joining of adjacent nucleotides - Using the diagram above, the vertical part (up and down) of the DNA molecule is formed when the sugar part (labelled Y) of one nucleotide bonds with the phosphate part (labelled Z) of an adjacent nucleotide. These bonds produce the sides of the ladder. The joining of the sugar/phosphate parts of adjacent nucleotide molecules causes the length of the new DNA molecule to increase. Two complete molecules of DNA are formed, identical to the original one. The diagram below shows how the sugar and the phosphate join.
3. Protein Synthesis – transcription then translation
A) Transcription - is the copying of the DNA molecule by mRNA
The 3 steps in Transcription: unzipping, Complementary base pairing and Joining of adjacent nucleotides
i. Unzipping
-A segment of DNA unzips. An enzyme (helicase) breaks the hydrogen bonds between the nitrogenous bases.
ii. Complementary Base Pairing
-RNA nucleotides bind with DNA nucleotides by complementary base pairing on one of the two DNA strands that are exposed after unzipping.
iii. Joining of Adjacent Nucleotides
An enzyme catalyzes the reaction that joins the sugar/phosphate molecules of adjacent nucleotides together.
-The newly produced mRNA strand passes from the nucleus into the cytoplasm to join with a ribosme.
B) Translation - mRNA codons are read, amino acids are brought to the mRNA strand and polypeptides (proteins) are produced at the ribosomes. Translation ends with the production of a protein.
The three specific parts of translation are:
i. Initiation - the ribosomal RNA (ribosome) and the mRNA strand become attached.
ii. Elongation - the polypeptide lengthens one amino acid at a time
-the tRNA anti-codons (3 letter code of the tRNA) bond to the mRNA codons (3 letter code of the mRNA) by complementary base pairing at the ribosome. The codons determine the type of amino acid brought to the polypeptide chain.
-The ribosome is large enough to accomodate two tRNA molecules at a time, one incoming and one outgoing The amino acid on the incoming tRNA forms a peptide bond with the amino acid on the outgoing tRNA.
-The ribosome then moves laterally, allowing the outgoing tRNA to leave and making space for a new incoming tRNA.
iii. Termination - occurs at the stop codon on the mRNA. The completed polypeptide dissociates from the ribosome
-Translation is shown in the diagram below. Be able to label the ribosomes (they look like snowmen, the mRNA strand (labeled X) and the polypeptide strand.
4. Determine the amino acids coded for by a strand of DNA
1. Determining codons from a strand of DNA
Consider the following strand of DNA read from left to right:
T T A T G C T C C T A A
When complementary base pairing occurs the following strand of mRNA is obtained.
A A U A C G A G G A U U
The codons are read three letters at a time from the transcribed mRNA. The codons are:
A A U A C G A G G A U U
2. Determining amino acids from mRNA codons
Find the codons in the table of mRNA codons below and the amino acids they code for.
AAU -Asparagine ACG - Threonine AGG - Arginine AUU -isoleucine
5 steps in protein synthesis
-DNA serves as a code for proteins
-Transcription occurs. One strand of DNA serves as a template (blueprint) for the formation of mRNA.
-after transcription, the newly formed mRNA goes into the cytoplasm and attaches to a ribosomes.
-tRNA which carry specific amino acids, have anticodons which bind complementarily with the mRNA codons
-The ribosome moves along the mRNA strand reading each codon. The tRNA anticodon bonds to it complementary codon. The amino acid carried by the tRNA is bonded to the polypeptide. The ribosome moves down the mRNA strand one codon at a time releasing a tRNA as it does and making room for another tRNA carrying an amino acid to join the mRNA.
a. DNA - carries the blueprint for the order of amino acids to be produced during protein synthesis.
b. Messenger RNA (mRNA) - copies the DNA molecule in the nucleus during transcription and goes into the cytoplasm and attaches itself to a ribosome. One 3 letter unit in an mRNA chain is called a codon.
c. Transfer RNA (tRNA) - drops off amino acids it is coded to carry at the ribsome. The 3 letter code is complementary to mRNA and this 3 letter code is called the anti-codon
d. Ribosome (rRNA) - this is where protein synthesis occurs. (Where the protein is assembled)
Recombinant DNA- contains DNA from 2 different sources. Organisms that have had a foreign gene inserted into them are called transgenic organisms
Use of Recombinant DNA research
a. in Bacteria - used to produce large amounts of protein, eg insulin, human growth hormone, and hepatitis vaccine.
b. in plants - genes transferred to cotton, corn, and potato strains have made these plants resistant to pests, because these cells produce an insect toxin.
-Plants have also been engineered to produce, hormones, clotting factors and antibodies
c. in animals. - genepharming using transgenic organisms to produce pharmaceuticals eg genes that code for therapeutic and diagnostic proteins are incorporated in and animal's DNA and appear in milk. There are plans to produce drugs for the treatment of cystic fibrosis, cancer, blood diseases, and other disorders. Antithroujmbi III is used for preventing blood clots in surgery is being produced by a herd of goats
DNA QUIZ QUESTIONS
1. What is the general name for chemicals like DNA and RNA
2. What general shape does the DNA molecule have?
3. List 4 differences between RNA and DNA
4. Describe the function of the mRNA in Protein Synthesis
5. What is the definition of a codon
6. What is the definition of an anti-codon
7. How many amino acids will result from the following strand of DNA?
A C G C C C A A A T A C
8. Name the two stages that make up protein synthesis
9. Where in the cell does replication take place?
10. Describe the function of the tRNA in Protein Synthesis?
11. Describe briefly the events that occur during transcription?
12. Name 2 common environmental mutagens
13. Describe briefly the events that occur during translation?
14. What is the definition of a) translation b) transcription
16. Define complementary base pairing and give an example?
17. Describe the function of the ribosome during protein synthesis
18. From a given strand of DNA. Show the results of Transcription or Replication
19. Make a drawing of DNA or RNA nucleotide and label the parts
20. Give 2 examples of a) purines b) pyrimidines
21. Name the 4 bases that make up DNA or RNA molecules
22. Describe how an environmental mutagen could cause a mutation
23. Describe briefly the events that occur during replication?
24. List 3 functions of DNA
25. Describe the function of the DNA in Protein Synthesis
26. What is the definition of a chromosomal mutation
27. What is the definition of a gene mutation
28. If the Nucleic acids are like ladders: What chemicals form the backbone of DNA or RNA molecules?
29. If the Nucleic acids are like ladders: What chemicals form the rungs of DNA or RNA molecule?
30. Briefly describe what occurs during Protein synthesis
31. What is recombinanant DNA. Give two uses of recombinant DNA
DNA SUBJECTIVE QUESTIONS
1. Describe 2 differences between the following pairs of terms
a. codon and anti codon b. replication and transcription c. RNA and DNA
d. transcription and translation e. chromosomal and gene mutation f. mRNA and tRNA
g. purine and pyrimidine h. complementary base pairing and joining of adjacent nucleotides
2. Describe complementary base pairing. Show an example.
3. Explain the roles/function of the following during Protein Synthesis:
a. DNA in the nucleus b. Transfer RNA (tRNA) c. Messenger RNA (mRNA) d. Ribosome
4. Name 2 environmental mutagens and describe how they could cause a mutation in an organism
5. Explain the process of DNA replication
6. Make a drawing of an RNA or DNA nucleotide and label the parts
7. Compare DNA and RNA with respect to the following things:
a. shape b. chemical makeup c. function d. abundance in the cell
8. Explain the process of translation
9. Explain the process of transcription
10. Describe in sequence, the process of protein synthesis. Include in your answer the names of the various steps, the organelles involved, and the names of the major molecules.
11. Describe how radiation or another such substance could cause mutations to occur.
12. From a given strand of DNA be able to determine the sequence of mRNA codons, and the amino acid strand produced.
Hot sheet questions
1. Describe the structure of DNA and RNA
2. Describe the steps in replication and transcription
3. Describe the function of the following during protein synthesis:
a. DNA b. mRNA c. tRNA d. rRNA e. ribosomes
4. Explain the process of Transcription
5. Briefly explain the process of translation
6. Describe the differences between RNA and DNA
7. Outline the process of protein synthesis